When performing Fluorescence measurements, in most applications, sensitivity is a key factor and at Sarspec we take it seriously. The configurations offered takes the sensitivity to a new level by using back thinned detectors with quantum efficiency up to 78% and 1 mm height pixel elements combined with large 200 µm slits and collecting lens. This combination makes all the difference in the detection of fluorescence signal.
Combining the detector with high power LEDs from LS-LED Light Source – with easily interchangeable LEDs – customers can have a sensitive and flexible solution for a fraction of the cost of a traditional spectrofluorimeter. For UV excitation we recommend the Xenon Flashlamp Light Source LS-XeF. The available pre-configured solutions from Sarspec for fluorescence analysis are:
FLUORESCENCE SPEC PACK / FLUORESCENCE SPEC PACK CUV
This is the most sensitive configuration available, mainly due to the sensitivity of the back thinned CCD array with high quantum efficiency (up to 78%) together with 200 µm slit, and collecting lens for the detector.
Also, the high power LEDs from the flexible LS-LED Light Source – where many different excitation wavelengths can be used by simply changing the LED slide – contributes greatly for the final sensitivity. In this configuration the sample holder takes an important role on the sensitivity and flexibility of the solution. Sarspec’s Multipurpose Cuvette Holder uses 12.7 mm diameter lens for large sample area illumination/collection and reflecting mirrors to employ all radiation available and supports a wide range of filters easily interchangeable.
FLUORESCENCE SPEC PACK / FLUORESCENCE SPEC PACK FLOW
In the Fluorescence SPEC PACK FLOW one can combine the flow analysis technique with fluorescence measurements making it a powerful tool for research in Biology and Chemistry.
The configuration of this solution consists on the highly sensitive SPEC SENSE+ FL spectrometer using a back thinned CCD (Q.E. = 78%) with collecting lens and 200 µm slit. The LS-LED light source with high power LEDs (where due to the cassette principle can easily change the excitation wavelength) the Flow Cell FCFPE or FCFSS with sapphire windows and SMA connection completes this solution.
FLUORESCENCE SPEC PACK / FLUORESCENCE SPEC PACK XEF
When high power excitation in the UV is required the light source is normally quite expensive. An affordable solution for users that want to work with UV excitation and obtain good results in Fluorescence is to use the Xenon Flashlamp Light Source LS-XeF from Sarspec. With it, it’s possible to obtain great fluorescence results with high flexibility.
The full configuration includes the high sensitivity spectrometer SPEC SENSE+ UV/Vis with a back-thinned CCD array with 2048 elements and great quantum efficiency in the UV Range, optical fibers with 1000 um core diameter (50 cm), the multipurpose cuvette holder CH-MP with filter slots, large lens and signal enhancing mirrors (CH-MP-EM).
FLUORESCENCE SPEC PACK / FLUORESCENCE SPEC PACK CUV BASIC
With great sensitivity in the Visible range, the SPEC PACK CUV BASIC is the most affordable solution for fluorescence available.
Using a SPEC STD FL spectrometer with 200 µm slit and collecting lens for greater sensitivity, the LS-LED Light Source with high power LEDs and where due to the LED slide principle can easily change the excitation wavelength, large core optical fibers and the flexible cuvette holder CH.
1. SPEC STD
| • Detection from 180-1100 w/ 2048 pixels linear detector CCD array | • Minimum integration time of 2 ms |
| • Triggering functions for easy integration (trigger in/out and delay) | • Portable, fast and small size |
| • High speed mini USB 2.0 for power and control | • Software LightScan included |
| • Also available as an OEM instrument | • SMA 905 fiber connector, no optical alignment required |
| • Robust: fully built in aluminium | • LED backlight indicator |
When looking for an affordable and flexible spectrometer the SPEC STD range is the best option. With detection from 180 to 1100 nm, it can be used in Absorbance, Transmittance, Reflectance, Irradiance, Color and Fluorescence measurements.
2. SPEC RES+
| • Detection from 180 to 1100 nm | • High speed mini USB 2.0 for power and control |
| • 3648 pixels linear array CCD detector | • Also available as an OEM instrument |
| • Great optical resolution of 0.2 nm in a standard configuration | • SMA 905 fiber connector, no optical alignment required |
| • Triggering functions for easy integration (trigger in/out and delay) | • Robust: fully built in alluminium |
| • Portable, fast and small size | • LED Backlight indicator |
For users looking for an affordable spectrometer with great resolution Sarspec offers the SPEC RES+ spectrometers range. With the best resolution/wavelength range ratio for a miniature CCD spectrometer the SPEC RES+ is the best option for Absorbance and Transmittance measurements as well for high throughput light source characterization.
3. SPEC SENSE+
| • Largest sensitive area, using 1000 µm height pixels | • Back thinned CCD array detector with 2048 pixels |
| • Detection from 200-1100 nm | • Great sensitivity and quantum efficiency up to 78% |
| • Great performance in the UV | • Triggering functions for easy integration and synchronization |
| • SMA 905 fiber connector, no optical alignment required | • High speed mini USB for full control of the spectrometer |
| • Software LightScan included | • Fully built in aluminium with LED backlight indicator |
Sarspec’s SPEC SENSE+ is a spectrometer specially built for low light level measurements such as Fluorescence and Raman but it also has the flexibility to be used in Absorbance, Transmittance, Irradiance and Reflectance measurements.
4. SPEC SPEED+
| • Fast acquisition rate up to 2857 spectra per second using burst mode | • 2 µs integration time with electronic shutter |
| • Back thinned CCD array detector with 2048 pixels | • Largest sensitive area, using 1000 µm height pixels |
| • Detection from 200-1100 nm | • Great sensitivity and quantum efficiency up to 78% |
| • Trigger functions for easy integration and synchronization | • SMA 905 fiber connector, no optical alignment required |
| • High speed mini USB for full control of the spectrometer | • Software LightScan+ Included |
| • Fully built in Alluminium with LED backlight indicator | |
With minimum integration time of 2 µs the SPEC SPEED+ is faster than any other spectrometer available using a CCD array. It was specially built for microseconds events including fast kinetics in chemical reactions, ignition processes and fluorescence monitoring among a large number of applications that need fast analysis.
